# ================================== # Manually curated genes transfert # ================================== * CONTACT: frederic.choulet@inra.fr; helene.rimbert@inra.fr; michael.alaux@inra.fr * The manually curated genes must be transfered in GFF3 format following The Sequence Ontology specifications. See description here : https://github.com/The-Sequence-Ontology/Specifications/blob/master/gff3.md * Please make sure your GFF3 file contains at least gene/mRNA/exon/CDS features with uniq ID and Parent attributes. The only case where an ID is not necessary concerns CDS & CDS features if they have Parent attribute defined. * All attributes and feature types are case sensitive! i.e. Id is not the same as ID. * Please make sure the chromosome names you are using are the same as the official release RefSeqV1 annotations and sequences. * Please, be as precise and complete as possible. Every feature which is not described in your annotation compared to the previous one will be lost. Here is an example of a minimal valid gff3 file: ##gff-version 3 chr1A IWGSCv1.0_UTR gene 102794 122504 51 + . ID=TraesCS1A01G000400;Name=NLR_1 chr1A IWGSCv1.0_UTR mRNA 102794 122504 . + . ID=TraesCS1A01G000400.1;Parent=TraesCS1A01G000400;Name=NLR_1.1 chr1A IWGSCv1.0_UTR exon 102794 104077 . + . ID=TraesCS1A01G000400.1.exon1;Parent=TraesCS1A01G000400.1 chr1A IWGSCv1.0_UTR five_prime_UTR 102794 104077 . + . Parent=TraesCS1A01G000400.1 chr1A IWGSCv1.0_UTR five_prime_UTR 120734 121262 . + . Parent=TraesCS1A01G000400.1 chr1A IWGSCv1.0_UTR exon 120734 122504 . + . Parent=TraesCS1A01G000400.1 chr1A IWGSCv1.0_UTR CDS 121263 121559 . + 0 Parent=TraesCS1A01G000400.1 chr1A IWGSCv1.0_UTR three_prime_UTR 121560 122504 . + . Parent=TraesCS1A01G000400.1